8–9 October 2026: Twinning on nucleic acid quantification methods with a focus on digital PCR, Rome, Italy

Based on the training needs identified across the network, molecular methods remain a high-priority topic, alongside sequencing and bioinformatics. This twinning initiative responds to that demand by providing practical insight into advanced nucleic acid quantification technologies and their applications in virology.

Activity overview

Over two days, participants will be introduced to the principles and applications of nucleic acid quantification methods, with emphasis on digital PCR as a reference method for accurate and reproducible absolute quantification.

The programme combines lectures, demonstrations, and practical laboratory sessions using different dPCR platforms based on droplet and microfluidic nanoplate technologies. Participants will gain experience in assay setup, workflow design, data analysis, interpretation of results, and examples of how dPCR can be applied in laboratory practice.

The visit will also provide opportunities for discussion on protocols, troubleshooting, implementation strategies, and future applications of the technology.

Target audience

The twinning activity is primarily intended for laboratory professionals and biologists working in molecular diagnostics, public health surveillance, or research involving viral pathogens. Applicants should have previous experience in molecular laboratory methods and an interest in implementing or strengthening quantitative nucleic acid technologies in their home institution.

Two participants will be selected through an application process.

Further details regarding the application procedure, eligibility criteria, and deadlines will be shared via email with Operational Contact Points (OCPs) and National focal points (NFPs).