NGS analyses

A number of different NGS-based analyses are offered by the Agency's special diagnostics service.

Whole genome sequencing of bacterial isolates

Whole genome sequencing of bacterial isolates is offered for contact tracing or issues relating to antibiotic resistance.

16S metagenomic sequencing

16S sequencing is carried out to determine which bacterial species are present in a clinical sample or isolate. The platform's method involves deep sequencing a 460 bp-long fragment of the 16S rRNA gene, with an automated method being used to analyse data. The result is a list of database hits and an estimated quantity compared with an internal standard.

Metagenomics for detection of an unknown pathogen

Metagenomic sequencing is offered in cases where infection is suspected but other methods have failed to identify a pathogen. All nucleic acid in the clinical sample is sequenced and data is analysed by a bioinformatician. The result is a technical report, a list of database hits and an interpretation of potential clinical relevance following consultation with a clinical microbiologist.

NGS bioinformatics

The NGS platform analyse data and develop software that is used by our microbiological operation. Two of these software products are available externally to customers.

SmiPrimer

Software to continually monitor recently published sequences in order to detect mutations that may affect the sensitivity of PCR-based diagnostics. By adding primer systems to the Agency's database, it is possible as an external customer to have them continually monitored and to have potential problems reported by e-mail.

SmiDesigner

Software for designing primers and probes for PCR-based methods.

Customer support

If you are interested in our services please contact our customer support.

For our contract customers, for referral of diagnostic samples please contact customer support during office hours (weekdays 08.00–16.30 CET) or after office hours our clinical microbiologist on duty on +46-10-205 24 00.